Новый метод анализа клеточного состава атеросклеротических бляшек

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Abstract

Objective. To study lymphocytic composition and lymphocyte state in atherosclerosis plaques for detection of immunologic mechanisms of their development and rupture.

Methods. We developed a protocol of immune cell release, located in plaque. The analysis of their state was performed with multicolor flow cytometry.

Results. It is shown that phenotypic composition of T-lymphocytes in plaque differs from the proper one in blood. CD4 T-cells and especially CD8 T-cells are highly activated in plaques; fraction of CD8 T-cells, expressing CD25 and HLA-DR, in plaques it was 10 times as great as in blood.

Conclusion. The analysis composition of T-cells in atherosclerosis plaques demonstrated that plaques represent different immunologic reservoir from blood that contains T-lymphocytes with high level of activation, related to antigen(s) in plaque that induces activation and cell migration. As a result, its possible to emphasize and characterize T-lymphocytes for following identification of their antigenic specificity.

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Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery