Новый метод анализа клеточного состава атеросклеротических бляшек
Abstract
Objective. To study lymphocytic composition and lymphocyte state in atherosclerosis plaques for detection of immunologic mechanisms of their development and rupture.Methods. We developed a protocol of immune cell release, located in plaque. The analysis of their state was performed with multicolor flow cytometry.
Results. It is shown that phenotypic composition of T-lymphocytes in plaque differs from the proper one in blood. CD4 T-cells and especially CD8 T-cells are highly activated in plaques; fraction of CD8 T-cells, expressing CD25 and HLA-DR, in plaques it was 10 times as great as in blood.
Conclusion. The analysis composition of T-cells in atherosclerosis plaques demonstrated that plaques represent different immunologic reservoir from blood that contains T-lymphocytes with high level of activation, related to antigen(s) in plaque that induces activation and cell migration. As a result, its possible to emphasize and characterize T-lymphocytes for following identification of their antigenic specificity.
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