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Comparison of the effectiveness of angiogenesis stimulationin the ischemic limbs of rats when delivering a combinationof VEGF and Ang genes in direct and cell-mediated ways

Authors: Samatoshenkov I.V.12, Alekseeva K.V.3, Zhuravleva M.N.4

Company: 1Kazan State Medical University, Ministry of Health of the Russian Federation, ulitsa Butlerova, 49, Kazan, 420012, Russian Federation;
2Interregional Clinical and Diagnostic Center, ulitsa Karbysheva, 12a, Kazan, 420101, Russian Federation;
3National Medical Research Center of Cardiology of the Ministry of Health of the Russian Federation, ulitsa Cherepkovskaya, 15a, Moscow, 121552, Russian Federation;
4Kazan (Volga Region) Federal University, ulitsa Kremlevskaya, 18, Kazan, 420008, Russian Federation

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Type:  Original articles


DOI: https://doi.org/10.24022/1997-3187-2019-13-3-250-262

For citation: Samatoshenkov I.V., Alekseeva K.V., Zhuravleva M.N. Comparison of the effectiveness of angiogenesis stimulation in the ischemic limbs of rats when delivering a combination of VEGF and Ang genes in direct and cell-mediated ways. Creative Cardiology. 2019; 13 (3): 250–62 (in Russ.). DOI: 10.24022/1997-3187-2019-13-3-250-262

Received / Accepted:  27.08.2019/04.09.2019

Keywords: calf muscle ischemia umbilical cord blood mononuclear cells angiogenesis vascular endothelial growth factor

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Abstract

Objective: in the model of chronic ischemia of the hind limb of the rat to investigate the effect on stimulation of angiogenesis adenovirus type V carrying the gene combination of recombinant human vascular endothelial growth factor VEGF 165 and Ang (Ad5-VEGF + Ad5-Ang), as in the case of direct injection of the gene construct, and with the help of mononuclear cells umbilical cord blood (HUCB-MNC).

Material and methods. The study was conducted on 45 Wistar rats. On day 14 after creation of ischemia by excision of the fragment of the femoral artery, the rats were divided into 3 groups: 1 – the group with gene combination – VEGF and Ang – injection at the distal part of the gastrocnemius muscle (group Ad5-VEGF + Ad5-Ang, n=15), 2 – the group with translation of the same gene combination of mononuclear cells in umbilical cord blood (group HUCB-MNC Ad5-VEGF + Ad5-Ang, n=15) and 3 – control group, with the saline injection in the same volume at the same points (NaCl group, n=15). On the 28th day after the genetic structures administration in the ischemic area, the ratio of capillaries/muscle fibers, the number of muscle fibers and the number of muscle fibers with a central location of nuclei (MCI) were estimated.

Results. In AD5-Ang and HUCB-MNCAd5-Ang groups observed a marked decrease in muscle fibers number, 2.1 and 1.7 times respectively (p<0.05). The ratio of the number of capillaries to the number of muscle fibers and the number of muscle fibers with centrally located nuclei increased in both experimental groups, but the difference was not significant.

Conclusion. The injection of genetic structures with adenovirus vector to a greater extent stimulates the regeneration of skeletal muscle, having a slight effect on angiogenesis in ischemia.

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About Authors

  • Igor’ V. Samatoshenkov, Postgraduate, Cardiac Surgeon, ORCID
  • Kseniya V. Alekseeva, Resident;
  • Margarita N. Zhuravleva, Junior Researcher, ORCID

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


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