Атеросклеротические бляшки в системе ex vivo

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Abstract

Objectives. To develop a model of culturing human atherosclerotic plaques ex vivo.

Materials and methods. Segments of coronary and carotid arteries with atherosclerotic plaques and normal arterial tissue were studied macroscopically and then dissected into small cubic blocks and large ring-shaped blocks. After that samples were cultured for 12-22 days in original culture medium. On the first day and every three days several samples of cultured tissue were analyzed histologically. For live/dead discrimination, cultured tissue samples were investigated with flow cytometry.

Results. We showed that tissue survival when cultured in small cubic blocks reduced to 8-12 day. According to the flow cytometry data the number of live cells was only 19 in 100 mg of cultured tissue. However, during cultivation of large ring-shaped blocks the survival of tissue was significantly higher. The integrity of endothelial layer and internal elastic membrane, the most sensitive to the changes in culture conditions, maintained for 20-22 days.

Conclusion. We have developed a method by which it became possible to cultivate the atherosclerotic plaque tissue ex vivo with preservation of the explants' architectonics during cultivation.

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Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery