Abstract
Objective. To determine of the nicotinamid adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent (respectively, NAD-and
NADP-dependent) dehydrogenases activity in platelets of aspirin-sensitive (ASP) and aspirin-resistant (ARP) patients with II–IV functional classes (FC)
stenocardia.
Material and methods. In 102 male patients with FC II–IV stenocardia aged 38–73 years were determined hemostasis on aspirin therapy at a dose of 75–150
mg/day. Platelet function was evaluated by optical aggregometry in determination of spontaneous and induced by adenosine diphosphate (ADP) platelet
aggregation. Depending on the suppression of platelet aggregation, all patients were divided into two groups: sensitive (n=48) and resistant (n=54) for aspirin.
The groups were matched on the number of patients with different functional classes. NAD- and NADP-dependent dehydrogenases activity in blood platelets
was determined by biochemiluminescence analyzer BCL 3606M (SCTB "Nauka", Krasnoyarsk). Studied the activity of the following enzymes: glucose-6-
phosphate dehydrogenase (Glu-6-pDH), glycerol-3-phosphate dehydrogenase (Gly-3-pDH), malic enzyme (ME), NAD and NADH-dependent reaction of
lactate dehydrogenase (LDH and NADH-LDH), NAD and NADH-dependent reaction of malate dehydrogenase (MDH and NADH-MDH), NAD-and
NADP-dependent glutamate dehydrogenase (NAD-GluDH and NADP-GluDH), NAD-and NADP-dependent isocitrate dehydrogenases (NAD-ICDH
and NADP-ICDH) and glutathione reductase (GluRed).
Results. In the study of the NAD-and NADP-dependent dehydrogenases activity platelets depending on stenocardia and sensitivity to aspirin found that III
FC ASP relative to control levels had increased activity of NADH-LDH, MDH and NADH NADH-GDH. Activity of GluRed in IV FC ARP increased relative
control parameters. Found an increase of the NADH-GDH activity in ASP of IV FC stenocardia relative to a reference range.
Conclusion. In patients with stenocardia of III–IV FC according to the sensitivity to aspirin revealed significant differences in the metabolism of platelets. The
most pronounced disorders of NAD(P)-dependent dehydrogenases activity in platelets found in IV FC ARP. It is shown that disturbances in the platelet
metabolism increase as functional class of stenocardia and are most pronounced in patients resistant to aspirin.
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