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Immunohystochemical analysis of collagen-I and collagen-III incidence in different forms of infective endocarditis: the role of underlying heart disease or the influence of infection?

Authors: Gryaznov D.V. 1,2, 2, Kovalev S.A. 1,2, 2, Zhdanov A.I. 2, Vorontsova Z.A. 1, Lavrenov A.L. 1, Pershin E.V. 3, Zolotareva S.N. 1, Kinshov A.N. 1, Nekrasova N.V. 1

Company: 1 Voronezh Burdenko State Medical University; Studencheskaya ulitsa, 10, Voronezh, 394036, Russian Federation;
2 Voronezh Regional Clinical Hospital #1 Moskovskiy prospect, 151, Voronezh, 394066, Russian Federation;
3 Voronezh Regional Bureau of Pathological Anatomy; Moskovskiy prospect, 151, Voronezh, 394066, Russian Federation

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Type:  Original articles


DOI: https://doi.org/10.24022/1997-3187-2018-12-2-100-119

For citation: Gryaznov D.V., Kovalev S.A., Zhdanov A.I., Vorontsova Z.A., Pershin E.V., Lavrenov A.L., Zolotareva S.N., Kinshov A.N., Nekrasova N.V. Immunohystochemical analysis of collagen-I and collagen-III incidence in different forms of infective endocarditis: the role of underlying heart disease or the influence of infection? Creative Cardiology. 2018; 12 (2): 100–19 (in Russ.). DOI: 10.24022/1997-3187-2018-12-2-100-119

Received / Accepted:  May 14, 2018 / May 21, 2018

Keywords: extracellular matrix infective endocarditis collagen-I collagen-III expression

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Abstract

Objective. Collagen-I (C-I) and Collagen-III (C-III) fibers are major components of heart valves extracellular matrix. The aim of study was to evaluate the influence of different forms of infective endocarditis (IE) on the expression of these proteins in human heart valves.

Methods. The data of 60 surgical patients with IE and 28 surgical patients with non-infective heart valve disease was compared. Immunohistochemistry with Anti-Collagen antibodies was performed. Protein expression was estimated via calculation of optical density of stain.

Results. Comparing general IE and control groups no difference in C-I expression was identified, and the level of C-III in IE group was significantly lower. Studying of collagen expression in subgroup with acute IE revealed the decrease of both C-I and C-III. In subacute IE the level of C-I was higher and the level of C-III was lower than in control group (p < 0.0001 in all comparisons). The lowest C-I and C-III level was found at subgroups with calcified aortic stenosis (CAS) (pC-I=0.003; pC-III=0.019) and at tricuspid valve (TV) samples (pC-I<0.0001; pC-III<0.0001). The level of both proteins was low in patients with secondary endocarditis with underlying CAS (pC-I<0.0001; pC-III<0.0001), C-III expression was also poor in cases of TV infective lesion (p<0.0001) and in mitral valve prolapse without and with IE (p=0.04; p=0.024).

Conclusion. The main IE hallmark was the decrease of C-III. The distribution of C-I and C-III in IE is under the influence of localization of valve lesion, underlying heart disease, form, activity and stage of the infection.

Acknowledgements. The study had no sponsorship.
Conflict of interest. The authors declare no conflict of interest.

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About Authors

Chief Editor

Leo A. Bockeria, MD, PhD, DSc, Professor, Academician of Russian Academy of Sciences, President of Bakoulev National Medical Research Center for Cardiovascular Surgery


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